The expression of two cell surface molecules were used to define the population of cells Treg.

First, the expression of two cell surface molecules were used to define the population of cells Treg, as such, they are CD4+ CD25+ CD4+ CD25+ Tregs. More recently, the transcription factor Foxp3 is identified, to exclusively expressed by Tregs and is known to in the conversion of in the conversion of CD4+ CD25 – T cells to CD4+ CD25+ Tregs.

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